Plant Tissue Culture Ppt | Pdf
Somatic hybridization (fusing two distinct species chemically or electrically). Immature or mature embryos
[ Stage 0: Selection ] ──> [ Stage 1: Initiation ] ──> [ Stage 2: Multiplication ] │ [ Stage 4: Acclimatization ] <── [ Stage 3: Rooting ] <─────────┘
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Using cell suspension cultures to produce medicinal compounds, pigments, and flavors. 7. Advantages and Limitations Advantages: Rapid multiplication of rare and endangered species. Production of disease-free, genetically identical clones. Independence from seasonal changes. Limitations: High costs of lab setup and specialized equipment. plant tissue culture ppt pdf
Used to produce haploid plants . These can be doubled using colchicine to create homozygous true-breeding lines rapidly, a massive shortcut for plant breeders.
Minimizing the duration of the callus phase; relying on direct organogenesis rather than indirect paths.
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High Auxin : Low Cytokinin = Low Auxin : High Cytokinin = Shoot development Balanced Auxin : Cytokinin = Unorganized cell mass (Callus) 5. Types of Plant Tissue Culture Techniques
Growing isolated roots to study root physiology or harvest root-specific compounds.
When you search for "plant tissue culture ppt pdf," you are typically looking for two different types of deliverables. Understanding the distinction helps you use them effectively. Key Applications of Plant Tissue Culture
Culture rooms with controlled temperature ( ), photoperiod, and light intensity. 3. Stages of Plant Tissue Culture The process is generally divided into several key stages:
Transitioning the fragile in vitro plantlets from high humidity and low light to greenhouse and field conditions. Plantlets are placed in porous soil mixes under misting systems so they can develop a protective waxy cuticle and functional stomata to survive on their own. Key Applications of Plant Tissue Culture